Chitosan-Based Delivery of Avian Reovirus Fusogenic Protein p10 Gene: In Vitro and In Vivo Studies towards a New Vaccine against Melanoma.

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  • Additional Information
    • Affiliation:
      Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, USACH, Alameda, 3363 Santiago, Chile
      Centro de Biotecnología Acuícola, Universidad de Santiago de Chile, USACH, Alameda, 3363 Santiago, Chile
      Centro de Nanociencias y Nanotecnología, Universidad de Santiago de Chile, USACH, Chile
      Centro de Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, USACH, Alameda, 3363 Santiago, Chile
      Departamento de Química de los Materiales, Facultad de Química y Biología, Universidad de Santiago de Chile, USACH, Alameda, 3363 Santiago, Chile
      Centro de Investigación Biomédica y Aplicada (CIBAP), Escuela de Medicina, Facultad de Ciencias Médicas, Universidad de Santiago de Chile, Chile
      Departamento de Salud, Universidad de Los Lagos, Osorno, Chile
      Laboratorio Biología Celular y Molecular, Instituto de Investigación en Ciencias Odontológicas, Facultad de Odontología, Universidad de Chile, Santiago, Chile
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      Reovirus is known to have an anticancer effect in both the preclinical and clinical assays. Current evidence suggests that the reovirus-mediated impact on tumor growth depends on the activation of specific antitumor immune responses. A feasible explanation for the oncolytic effects and immune system activation is through the expression of the fusogenic reovirus protein. In this work, we evaluated the in vivo antitumor effects of the expression of fusogenic protein p10 of avian reovirus (ARV-p10). We used chitosan nanoparticles (CH-NPs) as a vehicle for the ARV-p10 DNA in murine B16 melanoma models both in vitro and in vivo. We confirmed that ARV-p10 delivery through a chitosan-based formulation (ARV-p10 CH-NPs) was capable of inducing cell fusion in cultured melanoma cells, showing a mild cytotoxic effect. Interestingly, intratumor injection of ARV-p10 CH-NPs delayed tumor growth, without changing lymphoid populations in the tumor tissue and spleen. The injection of chitosan nanoparticles (CH-NPs) also delayed tumor growth, suggesting the nanoparticle itself would attack tumor cells. In conclusion, we proved that in vitro ARV-p10 protein expression using CH-NPs in murine melanoma cells induces a cytotoxic effect associated with its cell fusion. Further studies are necessary for establishing a protocol for efficient in vivo DNA delivery of fusion proteins to produce an antitumoral effect.
    • Journal Subset:
      Biomedical; Peer Reviewed; USA
    • ISSN:
      2314-6133
    • MEDLINE Info:
      NLM UID: 101600173
    • Publication Date:
      20200925
    • Publication Date:
      20200925
    • DOI:
      10.1155/2020/4045760
    • Accession Number:
      145973308
  • Citations
    • ABNT:
      ROBLES-PLANELLS, C. et al. Chitosan-Based Delivery of Avian Reovirus Fusogenic Protein p10 Gene: In Vitro and In Vivo Studies towards a New Vaccine against Melanoma. BioMed Research International, [s. l.], p. 1–11, 2020. DOI 10.1155/2020/4045760. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=145973308. Acesso em: 5 dez. 2020.
    • AMA:
      Robles-Planells C, Barrera-Avalos C, Rojo LE, et al. Chitosan-Based Delivery of Avian Reovirus Fusogenic Protein p10 Gene: In Vitro and In Vivo Studies towards a New Vaccine against Melanoma. BioMed Research International. September 2020:1-11. doi:10.1155/2020/4045760
    • APA:
      Robles-Planells, C., Barrera-Avalos, C., Rojo, L. E., Spencer, E., Cortez-San Martin, M., Matiacevich, S., Pavez, J., Milla, L. A., Navarro, F. D., Martínez, B. A., Bravo, F. J., Mella, A., Huidobro-Toro, J. P., Fernandez, R., Escobar, A., & Acuña-Castillo, C. (2020). Chitosan-Based Delivery of Avian Reovirus Fusogenic Protein p10 Gene: In Vitro and In Vivo Studies towards a New Vaccine against Melanoma. BioMed Research International, 1–11. https://doi.org/10.1155/2020/4045760
    • Chicago/Turabian: Author-Date:
      Robles-Planells, Claudia, Carlos Barrera-Avalos, Leonel E. Rojo, Eugenio Spencer, Marcelo Cortez-San Martin, Silvia Matiacevich, Jorge Pavez, et al. 2020. “Chitosan-Based Delivery of Avian Reovirus Fusogenic Protein P10 Gene: In Vitro and In Vivo Studies towards a New Vaccine against Melanoma.” BioMed Research International, September, 1–11. doi:10.1155/2020/4045760.
    • Harvard:
      Robles-Planells, C. et al. (2020) ‘Chitosan-Based Delivery of Avian Reovirus Fusogenic Protein p10 Gene: In Vitro and In Vivo Studies towards a New Vaccine against Melanoma’, BioMed Research International, pp. 1–11. doi: 10.1155/2020/4045760.
    • Harvard: Australian:
      Robles-Planells, C, Barrera-Avalos, C, Rojo, LE, Spencer, E, Cortez-San Martin, M, Matiacevich, S, Pavez, J, Milla, LA, Navarro, FD, Martínez, BA, Bravo, FJ, Mella, A, Huidobro-Toro, JP, Fernandez, R, Escobar, A & Acuña-Castillo, C 2020, ‘Chitosan-Based Delivery of Avian Reovirus Fusogenic Protein p10 Gene: In Vitro and In Vivo Studies towards a New Vaccine against Melanoma’, BioMed Research International, pp. 1–11, viewed 5 December 2020, .
    • MLA:
      Robles-Planells, Claudia, et al. “Chitosan-Based Delivery of Avian Reovirus Fusogenic Protein P10 Gene: In Vitro and In Vivo Studies towards a New Vaccine against Melanoma.” BioMed Research International, Sept. 2020, pp. 1–11. EBSCOhost, doi:10.1155/2020/4045760.
    • Chicago/Turabian: Humanities:
      Robles-Planells, Claudia, Carlos Barrera-Avalos, Leonel E. Rojo, Eugenio Spencer, Marcelo Cortez-San Martin, Silvia Matiacevich, Jorge Pavez, et al. “Chitosan-Based Delivery of Avian Reovirus Fusogenic Protein P10 Gene: In Vitro and In Vivo Studies towards a New Vaccine against Melanoma.” BioMed Research International, September 19, 2020, 1–11. doi:10.1155/2020/4045760.
    • Vancouver/ICMJE:
      Robles-Planells C, Barrera-Avalos C, Rojo LE, Spencer E, Cortez-San Martin M, Matiacevich S, et al. Chitosan-Based Delivery of Avian Reovirus Fusogenic Protein p10 Gene: In Vitro and In Vivo Studies towards a New Vaccine against Melanoma. BioMed Research International [Internet]. 2020 Sep 19 [cited 2020 Dec 5];1–11. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=145973308