Acidic Phospholipase A2-Peptide Derivative Modulates Oxidative Status and Microstructural Reorganization of Scar Tissue after Cutaneous Injury.

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  • Additional Information
    • Affiliation:
      Department of Animal Biology, Federal University of Viçosa, Viçosa, MG, Brazil
      Biomolecules Nucleus, Federal University of Viçosa, Viçosa, MG, Brazil
      Department of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
      Institute of Biomedical Science, Department of Structural Biology, Federal University of Alfenas, Alfenas 37130-001, MG, Brazil
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      From in vitro and in vivo models, the proliferative and healing potential of an acidic phospholipase A2 (LAPLA2) from Lachesis muta venom was investigated. The LAPLA2 proliferative activity was evaluated on fibroblasts and keratinocytes cultured, and the antioxidant and regenerative potential of LAPLA2 was analyzed in a murine model. The animal study consisted of four groups: C (negative control): 0.9% NaCl; SS (positive control): 1% silver sulfadiazine; L1 group: 0.5% LAPLA2; and L2 group: 0.25% LAPLA2. Wounds were topically treated daily for 12 days, and scar tissue samples were collected every 4 days. In vitro, LAPLA2 stimulated marked time-dependent cell proliferation. In vivo, it increased the antioxidant activity of superoxide dismutase (SOD) and catalase (CAT) and decreased malondialdehyde (MDA) and carbonyl protein (CP) levels in scar tissue treated with LAPLA2 at 0.5%. This peptide was effective in stimulating cellular proliferation, neoangiogenesis, type I and III collagen deposition, and maturation in a time-dependent-way, reducing the time required for wound closure. Our results indicated that LAPLA2 presented a remarkable potential in improving the oxidative status and microstructural reorganization of the scar tissue by stimulation of cellularity, angiogenesis, colagenogenesis, and wound contraction, suggesting that the peptide could be a potential candidate for a new healing drug.
    • Journal Subset:
      Alternative/Complementary Therapies; Biomedical; Europe; Peer Reviewed; UK & Ireland
    • ISSN:
      1741-427X
    • MEDLINE Info:
      NLM UID: 101215021
    • Publication Date:
      20200716
    • Publication Date:
      20200716
    • DOI:
      http://dx.doi.org/10.1155/2020/8273986
    • Accession Number:
      144527664
  • Citations
    • ABNT:
      GARCÍA, E. R. et al. Acidic Phospholipase A2-Peptide Derivative Modulates Oxidative Status and Microstructural Reorganization of Scar Tissue after Cutaneous Injury. Evidence-based Complementary & Alternative Medicine (eCAM), [s. l.], p. 1–13, 2020. DOI 10.1155/2020/8273986. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=144527664. Acesso em: 14 ago. 2020.
    • AMA:
      García ER, Barros E, Stransky S, et al. Acidic Phospholipase A2-Peptide Derivative Modulates Oxidative Status and Microstructural Reorganization of Scar Tissue after Cutaneous Injury. Evidence-based Complementary & Alternative Medicine (eCAM). July 2020:1-13. doi:10.1155/2020/8273986
    • APA:
      García, E. R., Barros, E., Stransky, S., Chávez-Olórtegui, C., Freitas, M. B., Novaes, R. D., & Gonçalves, R. V. (2020). Acidic Phospholipase A2-Peptide Derivative Modulates Oxidative Status and Microstructural Reorganization of Scar Tissue after Cutaneous Injury. Evidence-Based Complementary & Alternative Medicine (ECAM), 1–13. https://doi.org/10.1155/2020/8273986
    • Chicago/Turabian: Author-Date:
      García, Estefanny Ruiz, Edvaldo Barros, Stephanie Stransky, Carlos Chávez-Olórtegui, Mariella Bontempo Freitas, Rômulo Dias Novaes, and Reggiani Vilela Gonçalves. 2020. “Acidic Phospholipase A2-Peptide Derivative Modulates Oxidative Status and Microstructural Reorganization of Scar Tissue after Cutaneous Injury.” Evidence-Based Complementary & Alternative Medicine (ECAM), July, 1–13. doi:10.1155/2020/8273986.
    • Harvard:
      García, E. R. et al. (2020) ‘Acidic Phospholipase A2-Peptide Derivative Modulates Oxidative Status and Microstructural Reorganization of Scar Tissue after Cutaneous Injury’, Evidence-based Complementary & Alternative Medicine (eCAM), pp. 1–13. doi: 10.1155/2020/8273986.
    • Harvard: Australian:
      García, ER, Barros, E, Stransky, S, Chávez-Olórtegui, C, Freitas, MB, Novaes, RD & Gonçalves, RV 2020, ‘Acidic Phospholipase A2-Peptide Derivative Modulates Oxidative Status and Microstructural Reorganization of Scar Tissue after Cutaneous Injury’, Evidence-based Complementary & Alternative Medicine (eCAM), pp. 1–13, viewed 14 August 2020, .
    • MLA:
      García, Estefanny Ruiz, et al. “Acidic Phospholipase A2-Peptide Derivative Modulates Oxidative Status and Microstructural Reorganization of Scar Tissue after Cutaneous Injury.” Evidence-Based Complementary & Alternative Medicine (ECAM), July 2020, pp. 1–13. EBSCOhost, doi:10.1155/2020/8273986.
    • Chicago/Turabian: Humanities:
      García, Estefanny Ruiz, Edvaldo Barros, Stephanie Stransky, Carlos Chávez-Olórtegui, Mariella Bontempo Freitas, Rômulo Dias Novaes, and Reggiani Vilela Gonçalves. “Acidic Phospholipase A2-Peptide Derivative Modulates Oxidative Status and Microstructural Reorganization of Scar Tissue after Cutaneous Injury.” Evidence-Based Complementary & Alternative Medicine (ECAM), July 11, 2020, 1–13. doi:10.1155/2020/8273986.
    • Vancouver/ICMJE:
      García ER, Barros E, Stransky S, Chávez-Olórtegui C, Freitas MB, Novaes RD, et al. Acidic Phospholipase A2-Peptide Derivative Modulates Oxidative Status and Microstructural Reorganization of Scar Tissue after Cutaneous Injury. Evidence-based Complementary & Alternative Medicine (eCAM) [Internet]. 2020 Jul 11 [cited 2020 Aug 14];1–13. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=144527664