Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR.

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  • Additional Information
    • Affiliation:
      Pathology Department of Brasília University, Brasília, Federal District, Brazil
      Catholic University of Brasilia, Brasília, Federal District, Brazil
      Gynecological Unit, University Hospital of Brasilia, Brasília, Federal District, Brazil
      Molecular Biology Laboratory-LACEN DF, Brazil
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      Introduction. Despite increasing application of molecular diagnostic methods for the detection of sexually transmitted infections, the cytological findings in pap smears of patients with pathogens that can be identified only by PCR are not yet well described. The aim of this study was to describe the most common cytological features in cervical pap smears of patients with Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum detected by multiplex PCR. Methods. Cervical samples for conventional and liquid-based cytology and for multiplex PCR were collected from women ranging from 23 to 54 years old, who underwent routine screening at a gynecological Unit. Results. Multiplex PCR was positive in 36.2% of the samples: Ureaplasma parvum 14.9%, Chlamydia trachomatis 10.6%, Trichomonas vaginalis 10.6%, Mycoplasma hominis 8.5%, Ureaplasma urealyticum 4.2%, Neisseria gonorrhoeae 2.1%, and Mycoplasma genitalium (0). Multiple pathogens were observed in 12.8% of samples. Microscopic cervicitis (≥10 polymorphonuclear leukocytes/epithelial cell) and normal (predominantly lactobacillary) microbiota were the most frequent findings in the samples in which the pathogens were detected alone or in multiple infections, except for samples with Trichomonas vaginalis in which the coccobacillary microbiota was the most common. In samples with microscopic cervicitis and normal microbiota, those with at least one pathogen identified by multiplex PCR were significantly more frequent than those with no pathogen, 66.6% versus 33.3%. Conclusion. Failure to identify an inflammatory agent in pap smear with intense neutrophil exudate may suggest the presence of Ureaplasma parvum, Ureaplasma urealyticum, Chlamydia trachomatis, or Trichomonas vaginalis. A remark on the intensity of inflammation should be made in the reports of cervical pap smears so that this cytological finding can be correlated with clinical and PCR results.
    • Journal Subset:
      Biomedical; Peer Reviewed; USA
    • ISSN:
      2314-6133
    • MEDLINE Info:
      NLM UID: 101600173
    • Publication Date:
      20200711
    • Publication Date:
      20200711
    • DOI:
      10.1155/2020/7045217
    • Accession Number:
      144459939
  • Citations
    • ABNT:
      CARNEIRO, F. P. et al. Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR. BioMed Research International, [s. l.], p. 1–10, 2020. DOI 10.1155/2020/7045217. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=144459939. Acesso em: 28 set. 2020.
    • AMA:
      Carneiro FP, Darós AC, Darós ACM, et al. Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR. BioMed Research International. July 2020:1-10. doi:10.1155/2020/7045217
    • APA:
      Carneiro, F. P., Darós, A. C., Darós, A. C. M., de Castro, T. M. M. L., de Vasconcelos Carneiro, M., Fidelis, C. R., Vilioni, M. V., da Costa Matsunaga, M. E., Sidou, J. M. O., Chaves, M. A. L. D., Pereira, L. C., de Resende, C. N., de Castro Moreira dos Santos, A., Ferreira, V. M., & Motoyama, A. B. (2020). Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR. BioMed Research International, 1–10. https://doi.org/10.1155/2020/7045217
    • Chicago/Turabian: Author-Date:
      Carneiro, Fabiana Pirani, Andersen Charles Darós, Adriana Cysneiro Milhomem Darós, Tércia Maria Mendes Lousa de Castro, Marcos de Vasconcelos Carneiro, Cecília Ramos Fidelis, Mariane Vieira Vilioni, et al. 2020. “Cervical Cytology of Samples with Ureaplasma Urealyticum, Ureaplasma Parvum, Chlamydia Trachomatis, Trichomonas Vaginalis, Mycoplasma Hominis, and Neisseria Gonorrhoeae Detected by Multiplex PCR.” BioMed Research International, July, 1–10. doi:10.1155/2020/7045217.
    • Harvard:
      Carneiro, F. P. et al. (2020) ‘Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR’, BioMed Research International, pp. 1–10. doi: 10.1155/2020/7045217.
    • Harvard: Australian:
      Carneiro, FP, Darós, AC, Darós, ACM, de Castro, TMML, de Vasconcelos Carneiro, M, Fidelis, CR, Vilioni, MV, da Costa Matsunaga, ME, Sidou, JMO, Chaves, MALD, Pereira, LC, de Resende, CN, de Castro Moreira dos Santos, A, Ferreira, VM & Motoyama, AB 2020, ‘Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR’, BioMed Research International, pp. 1–10, viewed 28 September 2020, .
    • MLA:
      Carneiro, Fabiana Pirani, et al. “Cervical Cytology of Samples with Ureaplasma Urealyticum, Ureaplasma Parvum, Chlamydia Trachomatis, Trichomonas Vaginalis, Mycoplasma Hominis, and Neisseria Gonorrhoeae Detected by Multiplex PCR.” BioMed Research International, July 2020, pp. 1–10. EBSCOhost, doi:10.1155/2020/7045217.
    • Chicago/Turabian: Humanities:
      Carneiro, Fabiana Pirani, Andersen Charles Darós, Adriana Cysneiro Milhomem Darós, Tércia Maria Mendes Lousa de Castro, Marcos de Vasconcelos Carneiro, Cecília Ramos Fidelis, Mariane Vieira Vilioni, et al. “Cervical Cytology of Samples with Ureaplasma Urealyticum, Ureaplasma Parvum, Chlamydia Trachomatis, Trichomonas Vaginalis, Mycoplasma Hominis, and Neisseria Gonorrhoeae Detected by Multiplex PCR.” BioMed Research International, July 8, 2020, 1–10. doi:10.1155/2020/7045217.
    • Vancouver/ICMJE:
      Carneiro FP, Darós AC, Darós ACM, de Castro TMML, de Vasconcelos Carneiro M, Fidelis CR, et al. Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR. BioMed Research International [Internet]. 2020 Jul 8 [cited 2020 Sep 28];1–10. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=144459939