Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs.

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  • Additional Information
    • Affiliation:
      Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas, Avenida Brasil, 4365, CEP 21040-900, Manguinhos, Rio de Janeiro, RJ, Brazil
      Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Imunomodulação e Protozoologia, Avenida Brasil, 4365, CEP 21040-900, Manguinhos, Rio de Janeiro, RJ, Brazil
      Fundação Oswaldo Cruz, Centro de Desenvolvimento Tecnológico em Saúde, Avenida Brasil, 4365, CEP 21040-900, Manguinhos, Rio de Janeiro, RJ, Brazil
      Centro de Controle de Zoonoses-Prefeitura Municipal de Montes Claros-Avenida Antonio Lafetá Rebelo, 1371, CEP 39402-089, Santa Lucia II, Montes Claros-MG, Brazil
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      Leishmania spp. proteases have been proposed as virulence factors contributing to adaptive success these parasites to the mammalian hosts. Since these enzymes are poorly studied in naturally infected dogs, this work aims to show the differences in metalloprotease and cysteine proteases gene expression in ear edge skin of dogs naturally infected by Leishmania (Leishmania) infantum. A cohort of dogs (n = 20) naturally infected by L. (L.) infantum was clinically classified as asymptomatic, oligosymptomatic, and polysymptomatic and the parasite load range estimated. The analysis of proteases expression by RT-PCR in the ear edge skin was also assessed, suggesting more transcripts of proteases in cDNA samples from polysymptomatic dogs than oligosymptomatic and asymptomatic ones. Metalloprotease RT-PCR assays yielded products (202 bp) in all assessed cDNA dog samples. In contrast, cysteine proteases transcripts (227 bp) had shown to be better detected in cDNA samples of polysymptomatic dogs, compared with cDNA samples from asymptomatic and oligosymptomatic dogs. Predictive in silico assays suggested that secondary structures of metalloproteasee mRNAs can be more stable than cysteine proteases at the skin temperature of dogs. Evidence is presented that during natural infection of dogs by L. (L.) infantum, this parasite produces transcripts of metalloprotease and cysteine protease RNA in the skin from asymptomatic, oligosymptomatic, and polysymptomatic dogs.
    • Journal Subset:
      Biomedical; Peer Reviewed; USA
    • ISSN:
      2314-6133
    • MEDLINE Info:
      NLM UID: 101600173
    • Publication Date:
      20200701
    • Publication Date:
      20200701
    • DOI:
      http://dx.doi.org/10.1155/2020/2615787
    • Accession Number:
      144284831
  • Citations
    • ABNT:
      VELOSO, L. B. et al. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International, [s. l.], p. 1–8, 2020. DOI 10.1155/2020/2615787. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=144284831. Acesso em: 7 ago. 2020.
    • AMA:
      Veloso LB, de Oliveira Cardoso F, dos Santos Charret K, et al. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International. June 2020:1-8. doi:10.1155/2020/2615787
    • APA:
      Veloso, L. B., de Oliveira Cardoso, F., dos Santos Charret, K., de Sá Silva, M. P., de Castro Côrtes, L. M., da Silva Calabrese, K., da Silva, F. S., de Sousa, J. F., Rocha, M. F., & Alves, C. R. (2020). Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International, 1–8. https://doi.org/10.1155/2020/2615787
    • Chicago/Turabian: Author-Date:
      Veloso, Laura Barral, Flávia de Oliveira Cardoso, Karen dos Santos Charret, Matheus Pereira de Sá Silva, Luzia Monteiro de Castro Côrtes, Kátia da Silva Calabrese, Franklin Souza da Silva, Joel Fontes de Sousa, Marília Fonseca Rocha, and Carlos Roberto Alves. 2020. “Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) Infantum in Ear Edge Skin of Naturally Infected Dogs.” BioMed Research International, June, 1–8. doi:10.1155/2020/2615787.
    • Harvard:
      Veloso, L. B. et al. (2020) ‘Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs’, BioMed Research International, pp. 1–8. doi: 10.1155/2020/2615787.
    • Harvard: Australian:
      Veloso, LB, de Oliveira Cardoso, F, dos Santos Charret, K, de Sá Silva, MP, de Castro Côrtes, LM, da Silva Calabrese, K, da Silva, FS, de Sousa, JF, Rocha, MF & Alves, CR 2020, ‘Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs’, BioMed Research International, pp. 1–8, viewed 7 August 2020, .
    • MLA:
      Veloso, Laura Barral, et al. “Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) Infantum in Ear Edge Skin of Naturally Infected Dogs.” BioMed Research International, June 2020, pp. 1–8. EBSCOhost, doi:10.1155/2020/2615787.
    • Chicago/Turabian: Humanities:
      Veloso, Laura Barral, Flávia de Oliveira Cardoso, Karen dos Santos Charret, Matheus Pereira de Sá Silva, Luzia Monteiro de Castro Côrtes, Kátia da Silva Calabrese, Franklin Souza da Silva, Joel Fontes de Sousa, Marília Fonseca Rocha, and Carlos Roberto Alves. “Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) Infantum in Ear Edge Skin of Naturally Infected Dogs.” BioMed Research International, June 28, 2020, 1–8. doi:10.1155/2020/2615787.
    • Vancouver/ICMJE:
      Veloso LB, de Oliveira Cardoso F, dos Santos Charret K, de Sá Silva MP, de Castro Côrtes LM, da Silva Calabrese K, et al. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International [Internet]. 2020 Jun 28 [cited 2020 Aug 7];1–8. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=144284831