The First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with HLA Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Twelve Affected Siblings.

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  • Additional Information
    • Affiliation:
      Superior A.R.T., 1126/2 Vanit Building II, New Petchburi Road, Makkasan, Ratchathewi, Bangkok 10400, Thailand
      THAI StemLife, 566/3 Soi Ramkhamhaeng 39 (Thepleela 1), Prachaouthit Rd., Wangthonglang, Wangthonglang, Bangkok 10310, Thailand
      Hematopoietic Stem Cell Transplant Unit, Wattanosoth Hospital, 2 Soi Soonvijai 7, New Petchburi Road, Huaykwang, Bangkok 10310, Thailand
      Genea, 321 Kent Street, Sydney, New South Wales 2000, Australia
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      Background and Methods. In Southeast Asia and particularly in Thailand, β0-thalassemia/hemoglobin E (HbE) disease is a common hereditary hematological disease. In a retrospective analysis, we investigated the outcomes of blastocyst preimplantation genetic diagnosis (PGD) for α- and β-thalassemia exclusion, with human leukocyte antigen (PGD-HLA) matching, from 2007 to 2016 at Superior A.R.T. (Bangkok, Thailand) and subsequent hematopoietic stem cell transplantation (HSCT) treatment of affected siblings. The main outcome measures included the proportion of biopsied cycles with at least one screened embryo suitable for transfer, implantation rate, live birth rate, and curative HSCT post-PGD-HLA. Results. In 221 cycles from 138 patients (104 cycles requiring HLA matching), 90.5% had embryo(s) biopsied for genetic testing. There were 119 embryo transfers for thalassemia (76) and thalassemia-HLA cases (43), respectively, resulting in overall clinical pregnancy rates of 54.6%, implantation rates of 45.7%, and live birth rates of 44.1%. Our dataset included fifteen PGD-HLA live births with successful HSCT in twelve affected siblings, 67% using umbilical cord blood stem cells (UCBSC) as the only SC source. Conclusions. We report favorable thalassemia PGD and PGD-HLA laboratory and clinical outcomes from a single center. The ultimate success in PGD-HLA is of course the cure of a thalassemia-affected sibling by HSCT. Our PGD-HLA HSCT series is the first and largest performed entirely in Asia with twelve successful and two pending cures and predominant UCBSC use.
    • Journal Subset:
      Biomedical; Peer Reviewed; USA
    • ISSN:
      2314-6133
    • MEDLINE Info:
      NLM UID: 101600173
    • Publication Date:
      20200630
    • Publication Date:
      20200630
    • DOI:
      http://dx.doi.org/10.1155/2020/5292090
    • Accession Number:
      144260348
  • Citations
    • ABNT:
      TIEWSIRI, K. et al. The First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with HLA Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Twelve Affected Siblings. BioMed Research International, [s. l.], p. 1–10, 2020. DOI 10.1155/2020/5292090. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=144260348. Acesso em: 18 set. 2020.
    • AMA:
      Tiewsiri K, Manipalviratn S, Sutheesophon W, et al. The First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with HLA Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Twelve Affected Siblings. BioMed Research International. June 2020:1-10. doi:10.1155/2020/5292090
    • APA:
      Tiewsiri, K., Manipalviratn, S., Sutheesophon, W., Vanichsetakul, P., Thaijaroen, P., Ketcharoon, P., Bradley, C. K., McArthur, S. J., Krutsawad, W., Marshall, J. T. A., & Papadopoulos, K. I. (2020). The First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with HLA Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Twelve Affected Siblings. BioMed Research International, 1–10. https://doi.org/10.1155/2020/5292090
    • Chicago/Turabian: Author-Date:
      Tiewsiri, Kasorn, Somjate Manipalviratn, Warachaya Sutheesophon, Preeda Vanichsetakul, Piyarat Thaijaroen, Pagawadee Ketcharoon, Cara K. Bradley, et al. 2020. “The First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with HLA Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Twelve Affected Siblings.” BioMed Research International, June, 1–10. doi:10.1155/2020/5292090.
    • Harvard:
      Tiewsiri, K. et al. (2020) ‘The First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with HLA Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Twelve Affected Siblings’, BioMed Research International, pp. 1–10. doi: 10.1155/2020/5292090.
    • Harvard: Australian:
      Tiewsiri, K, Manipalviratn, S, Sutheesophon, W, Vanichsetakul, P, Thaijaroen, P, Ketcharoon, P, Bradley, CK, McArthur, SJ, Krutsawad, W, Marshall, JTA & Papadopoulos, KI 2020, ‘The First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with HLA Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Twelve Affected Siblings’, BioMed Research International, pp. 1–10, viewed 18 September 2020, .
    • MLA:
      Tiewsiri, Kasorn, et al. “The First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with HLA Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Twelve Affected Siblings.” BioMed Research International, June 2020, pp. 1–10. EBSCOhost, doi:10.1155/2020/5292090.
    • Chicago/Turabian: Humanities:
      Tiewsiri, Kasorn, Somjate Manipalviratn, Warachaya Sutheesophon, Preeda Vanichsetakul, Piyarat Thaijaroen, Pagawadee Ketcharoon, Cara K. Bradley, et al. “The First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with HLA Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Twelve Affected Siblings.” BioMed Research International, June 26, 2020, 1–10. doi:10.1155/2020/5292090.
    • Vancouver/ICMJE:
      Tiewsiri K, Manipalviratn S, Sutheesophon W, Vanichsetakul P, Thaijaroen P, Ketcharoon P, et al. The First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with HLA Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Twelve Affected Siblings. BioMed Research International [Internet]. 2020 Jun 26 [cited 2020 Sep 18];1–10. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=144260348