Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines.

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  • Additional Information
    • Affiliation:
      University Institute of Biochemistry and Biotechnology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan
      Department of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      Berberis lycium Royle has been traditionally used to cure rheumatism, eye and ear diseases, malarial fever, diabetes, stomach disorders, and skin diseases. There is a least amount of data available on cytotoxic capacity of Berberis lycium from Pakistani origin, so on this basis, the present study was aimed to screen Berberis lycium root bark extracts for cytotoxicity against cancer cell lines and isolation of chemical constituents from the most cytotoxic extract. Initial screening of extracts was performed on HepG2 cells at 100 μg/mL for 72 hours of treatment by using an MTT assay. Active fractions were subjected to a series of column chromatographies for the isolation of cytotoxic compounds. Molecular structures were elucidated by using combined data from 1H-NMR, 13C-NMR, and ESI-MS graphs. Assessment of reduction in cell proliferation by isolated compounds was performed on three human cancer cell lines (SK-Hep-1, HepG2, and NCI-H1299). Both n-hexane and chloroform fractions were found active with percent cell viabilities of 8.41 ± 2.23 and 22.31 ± 9.11 in HepG2 cells compared with lupeol 35.43 ± 3.35 percent viability. A protoberberine alkaloid identified as oxyberberine was isolated from chloroform fraction while β-sitosterol was isolated from n-hexane fraction. Oxyberberine inhibited SK-Hep-1 cell proliferation under a dose-dependent manner with an IC50 value of 34.26 ± 3.34 μM while HepG2 cells showed 50% inhibition at 62.96 ± 4.12 μM. β-Sitosterol showed reduction in cell viability in SK-Hep-1 cells and HepG2 cells with IC50 values of 123.12 ± 3.51 μM and 140 ± 4.21 μM. This is the first report on the isolation of oxyberberine and β-sitosterol from Berberis lycium root bark and their cytotoxic evaluation against SK-Hep-1 and NCI-H1299 cells. The cytotoxic potential of Berberis lycium Royle extracts and isolated compounds is suggesting that it is a promising candidate for anticancer drug discovery.
    • Journal Subset:
      Alternative/Complementary Therapies; Biomedical; Europe; Peer Reviewed; UK & Ireland
    • ISSN:
      1741-427X
    • MEDLINE Info:
      NLM UID: 101215021
    • Publication Date:
      20200620
    • Publication Date:
      20200620
    • DOI:
      http://dx.doi.org/10.1155/2020/2596082
    • Accession Number:
      143803516
  • Citations
    • ABNT:
      ANWAR, M. A. et al. Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines. Evidence-based Complementary & Alternative Medicine (eCAM), [s. l.], p. 1–9, 2020. DOI 10.1155/2020/2596082. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=143803516. Acesso em: 5 ago. 2020.
    • AMA:
      Anwar MA, Tabassam S, Gulfraz M, Sheeraz Ahmad M, Raja GK, Arshad M. Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines. Evidence-based Complementary & Alternative Medicine (eCAM). June 2020:1-9. doi:10.1155/2020/2596082.
    • AMA11:
      Anwar MA, Tabassam S, Gulfraz M, Sheeraz Ahmad M, Raja GK, Arshad M. Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines. Evidence-based Complementary & Alternative Medicine (eCAM). June 2020:1-9. doi:10.1155/2020/2596082
    • APA:
      Anwar, M. A., Tabassam, S., Gulfraz, M., Sheeraz Ahmad, M., Raja, G. K., & Arshad, M. (2020). Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines. Evidence-Based Complementary & Alternative Medicine (ECAM), 1–9. https://doi.org/10.1155/2020/2596082
    • Chicago/Turabian: Author-Date:
      Anwar, Muhammad Asad, Shahzadi Tabassam, Muhammad Gulfraz, Muhammad Sheeraz Ahmad, Ghazala Kaukab Raja, and Muhammad Arshad. 2020. “Isolation of Oxyberberine and β-Sitosterol from Berberis Lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines.” Evidence-Based Complementary & Alternative Medicine (ECAM), June, 1–9. doi:10.1155/2020/2596082.
    • Harvard:
      Anwar, M. A. et al. (2020) ‘Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines’, Evidence-based Complementary & Alternative Medicine (eCAM), pp. 1–9. doi: 10.1155/2020/2596082.
    • Harvard: Australian:
      Anwar, MA, Tabassam, S, Gulfraz, M, Sheeraz Ahmad, M, Raja, GK & Arshad, M 2020, ‘Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines’, Evidence-based Complementary & Alternative Medicine (eCAM), pp. 1–9, viewed 5 August 2020, .
    • MLA:
      Anwar, Muhammad Asad, et al. “Isolation of Oxyberberine and β-Sitosterol from Berberis Lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines.” Evidence-Based Complementary & Alternative Medicine (ECAM), June 2020, pp. 1–9. EBSCOhost, doi:10.1155/2020/2596082.
    • Chicago/Turabian: Humanities:
      Anwar, Muhammad Asad, Shahzadi Tabassam, Muhammad Gulfraz, Muhammad Sheeraz Ahmad, Ghazala Kaukab Raja, and Muhammad Arshad. “Isolation of Oxyberberine and β-Sitosterol from Berberis Lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines.” Evidence-Based Complementary & Alternative Medicine (ECAM), June 16, 2020, 1–9. doi:10.1155/2020/2596082.
    • Vancouver/ICMJE:
      Anwar MA, Tabassam S, Gulfraz M, Sheeraz Ahmad M, Raja GK, Arshad M. Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines. Evidence-based Complementary & Alternative Medicine (eCAM) [Internet]. 2020 Jun 16 [cited 2020 Aug 5];1–9. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=143803516