Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro.

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  • Additional Information
    • Affiliation:
      NMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany
      HOT Screen GmbH, Aspenhaustraße 25, 72770 Reutlingen, Germany
      University of Applied Sciences, Reutlingen, Alteburgstr. 150, 72762 Reutlingen, Germany
      Center for Ophthalmology, University Hospital Tübingen, Schleichstr. 12/1, 72076 Tübingen, Germany
      University of Tübingen, Geschwister-Scholl-Platz, 72074 Tübingen, Germany
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      It has been widely shown that biomaterial surface topography can modulate host immune response, but a fundamental understanding of how different topographies contribute to pro-inflammatory or anti-inflammatory responses is still lacking. To investigate the impact of surface topography on immune response, we undertook a systematic approach by analyzing immune response to eight grades of medical grade polyurethane of increasing surface roughness in three in vitro models of the human immune system. Polyurethane specimens were produced with defined roughness values by injection molding according to the VDI 3400 industrial standard. Specimens ranged from 0.1 μm to 18 μm in average roughness (Ra), which was confirmed by confocal scanning microscopy. Immunological responses were assessed with THP-1-derived macrophages, human peripheral blood mononuclear cells (PBMCs), and whole blood following culture on polyurethane specimens. As shown by the release of pro-inflammatory and anti-inflammatory cytokines in all three models, a mild immune response to polyurethane was observed, however, this was not associated with the degree of surface roughness. Likewise, the cell morphology (cell spreading, circularity, and elongation) in THP-1-derived macrophages and the expression of CD molecules in the PBMC model on T cells (HLA-DR and CD16), NK cells (HLA-DR), and monocytes (HLA-DR, CD16, CD86, and CD163) showed no influence of surface roughness. In summary, this study shows that modifying surface roughness in the micrometer range on polyurethane has no impact on the pro-inflammatory immune response. Therefore, we propose that such modifications do not affect the immunocompatibility of polyurethane, thereby supporting the notion of polyurethane as a biocompatible material.
    • Journal Subset:
      Biomedical; Peer Reviewed; USA
    • ISSN:
      2314-6133
    • MEDLINE Info:
      NLM UID: 101600173
    • Publication Date:
      20200515
    • Publication Date:
      20200515
    • DOI:
      10.1155/2020/3481549
    • Accession Number:
      143172290
  • Citations
    • ABNT:
      SEGAN, S. et al. Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro. BioMed Research International, [s. l.], p. 1–15, 2020. DOI 10.1155/2020/3481549. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=143172290. Acesso em: 24 out. 2020.
    • AMA:
      Segan S, Jakobi M, Khokhani P, et al. Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro. BioMed Research International. May 2020:1-15. doi:10.1155/2020/3481549
    • APA:
      Segan, S., Jakobi, M., Khokhani, P., Klimosch, S., Billing, F., Schneider, M., Martin, D., Metzger, U., Biesemeier, A., Xiong, X., Mukherjee, A., Steuer, H., Keller, B.-M., Joos, T., Schmolz, M., Rothbauer, U., Hartmann, H., Burkhardt, C., Lorenz, G., & Schneiderhan-Marra, N. (2020). Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro. BioMed Research International, 1–15. https://doi.org/10.1155/2020/3481549
    • Chicago/Turabian: Author-Date:
      Segan, Sören, Meike Jakobi, Paree Khokhani, Sascha Klimosch, Florian Billing, Markus Schneider, Dagmar Martin, et al. 2020. “Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in Vitro.” BioMed Research International, May, 1–15. doi:10.1155/2020/3481549.
    • Harvard:
      Segan, S. et al. (2020) ‘Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro’, BioMed Research International, pp. 1–15. doi: 10.1155/2020/3481549.
    • Harvard: Australian:
      Segan, S, Jakobi, M, Khokhani, P, Klimosch, S, Billing, F, Schneider, M, Martin, D, Metzger, U, Biesemeier, A, Xiong, X, Mukherjee, A, Steuer, H, Keller, B-M, Joos, T, Schmolz, M, Rothbauer, U, Hartmann, H, Burkhardt, C, Lorenz, G & Schneiderhan-Marra, N 2020, ‘Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro’, BioMed Research International, pp. 1–15, viewed 24 October 2020, .
    • MLA:
      Segan, Sören, et al. “Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in Vitro.” BioMed Research International, May 2020, pp. 1–15. EBSCOhost, doi:10.1155/2020/3481549.
    • Chicago/Turabian: Humanities:
      Segan, Sören, Meike Jakobi, Paree Khokhani, Sascha Klimosch, Florian Billing, Markus Schneider, Dagmar Martin, et al. “Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in Vitro.” BioMed Research International, May 12, 2020, 1–15. doi:10.1155/2020/3481549.
    • Vancouver/ICMJE:
      Segan S, Jakobi M, Khokhani P, Klimosch S, Billing F, Schneider M, et al. Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro. BioMed Research International [Internet]. 2020 May 12 [cited 2020 Oct 24];1–15. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=143172290