Manuka honey modulates the release profile of a dHL-60 neutrophil model under anti-inflammatory stimulation.

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  • Additional Information
    • Affiliation:
      Department of Biomedical Engineering, University of Memphis, 330 Engineering Technology Building, Memphis, TN, 38152, USA
      Division of Epidemiology, Biostatistics, and Environmental Health, School of Public Health, University of Memphis, 222 Robison Hall, Memphis, TN, 38152, USA
      Department of Orthopaedic Surgery & Biomedical Engineering, University of Tennessee Health Science Center, E228A Coleman Building, 956 Court Avenue, Memphis, TN, 38163, USA
      Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 201 Molecular Science Building, 858 Madison Ave, Memphis, TN, 38152, USA
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      Manuka honey, a wound treatment used to eradicate bacteria, resolve inflammation, and promote wound healing, is a current focus in the tissue engineering community as a tissue template additive. However, Manuka honey's effect on neutrophils during the inflammation-resolving phase has yet to be examined. This study investigates the effect of 0.5% and 3% Manuka honey on the release of cytokines, chemokines, and matrix-degrading enzymes from a dHL-60 neutrophil model in the presence of anti-inflammatory stimuli (TGF-β, IL-4, IL-4 +IL-13). We hypothesized that Manuka honey would reduce the output of pro-inflammatory signals and increase the release of anti-inflammatory signals. The results of this study indicate that 0.5% honey significantly increases the release of CXCL8/IL-8, CCL2/MCP-1, CCL4/MIP-1β, CCL20/MIP-3α, IL-4, IL-1ra, and FGF-13 while reducing Proteinase 3 release in the anti-inflammatory-stimulated models. However, 3% honey significantly increased the release of TNF-α and CXCL8/IL-8 while reducing the release of all other analytes. We replicated a subset of the most notable findings in primary human neutrophils, and the consistent results indicate that the HL-60 data are relevant to the performance of primary cells. These findings demonstrate the variable effects of Manuka honey on the release of cytokines, chemokines, and matrix-degrading enzymes of this model of neutrophil anti-inflammatory activity. This study reinforces the importance of tailoring the concentration of Manuka honey in a wound or tissue template to elicit the desired effects during the inflammation-resolving phase of wound healing. Future in vivo investigation should be undertaken to translate these results to a physiologically-relevant wound environment. Image 1 • Manuka honey alters the secretome of an anti-inflammatory dHL-60 neutrophil model. • 0.5% honey increases both pro-inflammatory and anti-inflammatory signal release. • 3% honey reduces all release except TNF-α and IL-8. • In primary human neutrophils, 0.5% honey increases IL-1ra, VEGF, and MMP-9.
    • Journal Subset:
      Double Blind Peer Reviewed; Europe; Nursing; Peer Reviewed; UK & Ireland
    • ISSN:
      0965-206X
    • MEDLINE Info:
      NLM UID: 9306822
    • Grant Information:
      . Research was supported by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under Award Number R15EB022345.
    • Publication Date:
      20200426
    • Publication Date:
      20200508
    • DOI:
      10.1016/j.jtv.2020.03.005
    • Accession Number:
      142853448
  • Citations
    • ABNT:
      MINDEN-BIRKENMAIER, B. A. et al. Manuka honey modulates the release profile of a dHL-60 neutrophil model under anti-inflammatory stimulation. Journal of Tissue Viability, [s. l.], v. 29, n. 2, p. 91–99, 2020. DOI 10.1016/j.jtv.2020.03.005. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=142853448. Acesso em: 6 dez. 2020.
    • AMA:
      Minden-Birkenmaier BA, Meadows MB, Cherukuri K, et al. Manuka honey modulates the release profile of a dHL-60 neutrophil model under anti-inflammatory stimulation. Journal of Tissue Viability. 2020;29(2):91-99. doi:10.1016/j.jtv.2020.03.005
    • APA:
      Minden-Birkenmaier, B. A., Meadows, M. B., Cherukuri, K., Smeltzer, M. P., Smith, R. A., Radic, M. Z., & Bowlin, G. L. (2020). Manuka honey modulates the release profile of a dHL-60 neutrophil model under anti-inflammatory stimulation. Journal of Tissue Viability, 29(2), 91–99. https://doi.org/10.1016/j.jtv.2020.03.005
    • Chicago/Turabian: Author-Date:
      Minden-Birkenmaier, Benjamin A., Meghan B. Meadows, Kasyap Cherukuri, Matthew P. Smeltzer, Richard A. Smith, Marko Z. Radic, and Gary L. Bowlin. 2020. “Manuka Honey Modulates the Release Profile of a DHL-60 Neutrophil Model under Anti-Inflammatory Stimulation.” Journal of Tissue Viability 29 (2): 91–99. doi:10.1016/j.jtv.2020.03.005.
    • Harvard:
      Minden-Birkenmaier, B. A. et al. (2020) ‘Manuka honey modulates the release profile of a dHL-60 neutrophil model under anti-inflammatory stimulation’, Journal of Tissue Viability, 29(2), pp. 91–99. doi: 10.1016/j.jtv.2020.03.005.
    • Harvard: Australian:
      Minden-Birkenmaier, BA, Meadows, MB, Cherukuri, K, Smeltzer, MP, Smith, RA, Radic, MZ & Bowlin, GL 2020, ‘Manuka honey modulates the release profile of a dHL-60 neutrophil model under anti-inflammatory stimulation’, Journal of Tissue Viability, vol. 29, no. 2, pp. 91–99, viewed 6 December 2020, .
    • MLA:
      Minden-Birkenmaier, Benjamin A., et al. “Manuka Honey Modulates the Release Profile of a DHL-60 Neutrophil Model under Anti-Inflammatory Stimulation.” Journal of Tissue Viability, vol. 29, no. 2, May 2020, pp. 91–99. EBSCOhost, doi:10.1016/j.jtv.2020.03.005.
    • Chicago/Turabian: Humanities:
      Minden-Birkenmaier, Benjamin A., Meghan B. Meadows, Kasyap Cherukuri, Matthew P. Smeltzer, Richard A. Smith, Marko Z. Radic, and Gary L. Bowlin. “Manuka Honey Modulates the Release Profile of a DHL-60 Neutrophil Model under Anti-Inflammatory Stimulation.” Journal of Tissue Viability 29, no. 2 (May 2020): 91–99. doi:10.1016/j.jtv.2020.03.005.
    • Vancouver/ICMJE:
      Minden-Birkenmaier BA, Meadows MB, Cherukuri K, Smeltzer MP, Smith RA, Radic MZ, et al. Manuka honey modulates the release profile of a dHL-60 neutrophil model under anti-inflammatory stimulation. Journal of Tissue Viability [Internet]. 2020 May [cited 2020 Dec 6];29(2):91–9. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=142853448