Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high-risk region of China.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Affiliation:
      Cancer Registry Office, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing China
      Department of Cancer Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing China
      Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda Maryland
      Promegene Translational Research Institute, Shenzhen China
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      Background: Little is known about the microbiota and upper gastrointestinal tumors. Esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcinoma (GCA) occur in adjacent organs, co-occur geographically, and share many risk factors despite being of different tissue types.Methods: This study characterized the microbial communities of paired tumor and nontumor samples from 67 patients with ESCC and 36 patients with GCA in Henan, China. DNA was extracted with the MoBio PowerSoil kit. The V4 region of the 16S ribosomal RNA gene was sequenced with MiniSeq and was processed with Quantitative Insights Into Microbial Ecology 1. The linear discriminant analysis effect size method was used to identify differentially abundant microbes, the Wilcoxon rank-sum test was used to test α diversity differences, and permutational multivariate analysis of variance was used to test for differences in β diversity.Results: The microbial environments of ESCC and GCA tissues were all composed primarily of Firmicutes, Bacteroidetes, and Proteobacteria. ESCC tumor tissues contained more Fusobacterium (3.2% vs 1.3%) and less Streptococcus (12.0% vs 30.2%) than nontumor tissues. GCA nontumor tissues had a greater abundance of Helicobacter (60.5% vs 11.8%), which may have been linked to the lower α diversity (58.0 vs 102.5; P = .0012) in comparison with tumor tissues. A comparison of ESCC and GCA nontumor tissues showed that the microbial composition (P = .0040) and the α diversity (87.0 vs 58.0; P = .00052) were significantly different. No significant differences were detected for α diversity within ESCC and GCA tumor tissues.Conclusions: This study showed differences in the microbial compositions of paired ESCC and GCA tumor and nontumor tissues and differences by organ site. Large-scale, prospective cohort studies are needed to confirm these findings.
    • Journal Subset:
      Biomedical; Peer Reviewed; USA
    • ISSN:
      0008-543X
    • MEDLINE Info:
      PMID: NLM31355925 NLM UID: 0374236
    • Grant Information:
      Z99 CA999999//Intramural NIH HHS/United States; 31570116//National Natural Science Foundation of China/International; 2016-I2M-3-001//Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences (CIFMS)/International; //Intramural Research Program of the National Cancer Institute of the National Institutes of Health/International; 2016YFC0901400//National Key Research and Development Program of precision medicine, China/International
    • Publication Date:
      20200513
    • Publication Date:
      20200612
    • DOI:
      http://dx.doi.org/10.1002/cncr.32403
    • Accession Number:
      139372867
  • Citations
    • ABNT:
      SHAO, D. et al. Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high-risk region of China. Cancer (0008543X), [s. l.], v. 125, n. 22, p. 3993–4002, 2019. DOI 10.1002/cncr.32403. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=139372867. Acesso em: 7 ago. 2020.
    • AMA:
      Shao D, Vogtmann E, Liu A, et al. Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high-risk region of China. Cancer (0008543X). 2019;125(22):3993-4002. doi:10.1002/cncr.32403
    • APA:
      Shao, D., Vogtmann, E., Liu, A., Qin, J., Chen, W., Abnet, C. C., & Wei, W. (2019). Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high-risk region of China. Cancer (0008543X), 125(22), 3993–4002. https://doi.org/10.1002/cncr.32403
    • Chicago/Turabian: Author-Date:
      Shao, Dantong, Emily Vogtmann, Anqi Liu, Junjie Qin, Wen Chen, Christian C. Abnet, and Wenqiang Wei. 2019. “Microbial Characterization of Esophageal Squamous Cell Carcinoma and Gastric Cardia Adenocarcinoma from a High-Risk Region of China.” Cancer (0008543X) 125 (22): 3993–4002. doi:10.1002/cncr.32403.
    • Harvard:
      Shao, D. et al. (2019) ‘Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high-risk region of China’, Cancer (0008543X), 125(22), pp. 3993–4002. doi: 10.1002/cncr.32403.
    • Harvard: Australian:
      Shao, D, Vogtmann, E, Liu, A, Qin, J, Chen, W, Abnet, CC & Wei, W 2019, ‘Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high-risk region of China’, Cancer (0008543X), vol. 125, no. 22, pp. 3993–4002, viewed 7 August 2020, .
    • MLA:
      Shao, Dantong, et al. “Microbial Characterization of Esophageal Squamous Cell Carcinoma and Gastric Cardia Adenocarcinoma from a High-Risk Region of China.” Cancer (0008543X), vol. 125, no. 22, Nov. 2019, pp. 3993–4002. EBSCOhost, doi:10.1002/cncr.32403.
    • Chicago/Turabian: Humanities:
      Shao, Dantong, Emily Vogtmann, Anqi Liu, Junjie Qin, Wen Chen, Christian C. Abnet, and Wenqiang Wei. “Microbial Characterization of Esophageal Squamous Cell Carcinoma and Gastric Cardia Adenocarcinoma from a High-Risk Region of China.” Cancer (0008543X) 125, no. 22 (November 15, 2019): 3993–4002. doi:10.1002/cncr.32403.
    • Vancouver/ICMJE:
      Shao D, Vogtmann E, Liu A, Qin J, Chen W, Abnet CC, et al. Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high-risk region of China. Cancer (0008543X) [Internet]. 2019 Nov 15 [cited 2020 Aug 7];125(22):3993–4002. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=139372867