High prevalence of class 1 integrons in clinical isolates of methicillin-resistant Staphylococcus aureus from India.

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    • Abstract:
      Introduction: Class1 integrons are one of the prevalent mechanisms of antibiotic resistance gene transfer in Gram-negative organisms, but their prevalence and role in the spread of antibiotic resistance genes in methicillin-resistant Staphylococcus aureus (MRSA) is unexplored. The purpose of this study was to investigate the prevalence of class 1 integrons in clinical isolates of MRSA.Materials and Methods: Total 143 MRSA isolates obtained from two different cities in India (Pune and Mumbai) were characterized by biochemical tests, and the antibiotic sensitivity was performed using the Clinical and Laboratory Standards Institute (CLSI) guidelines. The presence of class 1 integrons, sul1/qacE0Δ1 region of class 1 integron and mecA gene among these isolates was determined by polymerase chain reaction (PCR).Results: All 143 isolates were mecA positive and coagulase-positive. Overall, 71% of the MRSA isolates carried class 1 integrons; 58% (45/77) of the isolates obtained from Mumbai and 85% (56/66) of the isolates from Pune carried class 1 integrons. In all, 39% of these isolates carried sul1/qacEΔ1 region, thus confirming the association of class 1 integrons with antibiotic resistance genes. Along with β-lactam antibiotics the MRSA isolates were resistant to several other antibiotics, with resistance to erythromycin, ciprofloxacin and trimethoprim-sulfamethoxazole being observed in 75%, 66% and 60% of the isolates, respectively.Conclusion: To the best of our knowledge, this is the first report of class 1 integrons in MRSA isolates from India. The study provides insights into the prevalence of a novel mechanism adapted by MRSA for the propagation of antibiotic resistance genes.
    • Journal Subset:
      Asia; Biomedical
    • Special Interest:
      Laboratory Diagnosis
    • ISSN:
      0255-0857
    • MEDLINE Info:
      PMID: NLM25865973 NLM UID: 8700903
    • Publication Date:
      20150923
    • Publication Date:
      20151224
    • DOI:
      http://dx.doi.org/10.4103/0255-0857.154905
    • Accession Number:
      109720634
  • Citations
    • ABNT:
      MARATHE, N. P. et al. High prevalence of class 1 integrons in clinical isolates of methicillin-resistant Staphylococcus aureus from India. Indian Journal of Medical Microbiology, [s. l.], v. 33, n. 2, p. 231–236, 2015. DOI 10.4103/0255-0857.154905. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=109720634. Acesso em: 3 ago. 2020.
    • AMA:
      Marathe NP, Nagarkar SS, Vaishampayan AA, et al. High prevalence of class 1 integrons in clinical isolates of methicillin-resistant Staphylococcus aureus from India. Indian Journal of Medical Microbiology. 2015;33(2):231-236. doi:10.4103/0255-0857.154905.
    • AMA11:
      Marathe NP, Nagarkar SS, Vaishampayan AA, et al. High prevalence of class 1 integrons in clinical isolates of methicillin-resistant Staphylococcus aureus from India. Indian Journal of Medical Microbiology. 2015;33(2):231-236. doi:10.4103/0255-0857.154905
    • APA:
      Marathe, N. P., Nagarkar, S. S., Vaishampayan, A. A., Rasane, M. H., Samant, S. A., Dohe, V., Kagal, A., Shouche, Y. S., & Deshpande, N. (2015). High prevalence of class 1 integrons in clinical isolates of methicillin-resistant Staphylococcus aureus from India. Indian Journal of Medical Microbiology, 33(2), 231–236. https://doi.org/10.4103/0255-0857.154905
    • Chicago/Turabian: Author-Date:
      Marathe, N P, S S Nagarkar, A A Vaishampayan, M H Rasane, S A Samant, V Dohe, A Kagal, Y S Shouche, and N Deshpande. 2015. “High Prevalence of Class 1 Integrons in Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus from India.” Indian Journal of Medical Microbiology 33 (2): 231–36. doi:10.4103/0255-0857.154905.
    • Harvard:
      Marathe, N. P. et al. (2015) ‘High prevalence of class 1 integrons in clinical isolates of methicillin-resistant Staphylococcus aureus from India’, Indian Journal of Medical Microbiology, 33(2), pp. 231–236. doi: 10.4103/0255-0857.154905.
    • Harvard: Australian:
      Marathe, NP, Nagarkar, SS, Vaishampayan, AA, Rasane, MH, Samant, SA, Dohe, V, Kagal, A, Shouche, YS & Deshpande, N 2015, ‘High prevalence of class 1 integrons in clinical isolates of methicillin-resistant Staphylococcus aureus from India’, Indian Journal of Medical Microbiology, vol. 33, no. 2, pp. 231–236, viewed 3 August 2020, .
    • MLA:
      Marathe, N. P., et al. “High Prevalence of Class 1 Integrons in Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus from India.” Indian Journal of Medical Microbiology, vol. 33, no. 2, Apr. 2015, pp. 231–236. EBSCOhost, doi:10.4103/0255-0857.154905.
    • Chicago/Turabian: Humanities:
      Marathe, N P, S S Nagarkar, A A Vaishampayan, M H Rasane, S A Samant, V Dohe, A Kagal, Y S Shouche, and N Deshpande. “High Prevalence of Class 1 Integrons in Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus from India.” Indian Journal of Medical Microbiology 33, no. 2 (April 2015): 231–36. doi:10.4103/0255-0857.154905.
    • Vancouver/ICMJE:
      Marathe NP, Nagarkar SS, Vaishampayan AA, Rasane MH, Samant SA, Dohe V, et al. High prevalence of class 1 integrons in clinical isolates of methicillin-resistant Staphylococcus aureus from India. Indian Journal of Medical Microbiology [Internet]. 2015 Apr [cited 2020 Aug 3];33(2):231–6. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=109720634