Nuclear localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers.

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  • Additional Information
    • Source:
      Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8205768 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4644 (Electronic) Linking ISSN: 07302312 NLM ISO Abbreviation: J. Cell. Biochem.
    • Publication Information:
      Publication: <2004>- : Hoboken, NJ : Wiley-Liss
      Original Publication: New York : Liss, c1982-
    • Abstract:
      Smoothened (SMO) is a significant signalling protein which functions as a key transducer for the hedgehog signalling pathway, an important signalling mechanism with key roles in development and oncogenesis. The correlation of expression dynamics of SMO with pancreatic and colorectal cancer genesis has been known but with ambiguity. Therefore, in this study, we investigated messenger RNA (mRNA) and protein expression of SMO in pancreatic and colorectal cancers in our population and assessed relationship with various clinicopathological parameters. Surgically resected tumour and adjacent histologically normal tissues from 33 and 61 pancreatic and colorectal cancer patients were investigated in the present study. Expression of SMO was analysed by quantitative real-time polymerase chain reaction and immunohistochemistry. At mRNA level, SMO was overexpressed in 72.72% (24 of 33) and 50.81% (31 of 61) of the pancreatic and colorectal cancer cases as compared with their adjacent normal tissues. SMO immunohistochemical analysis revealed nuclear localization and overexpression was observed in 51.51% (17 of 33) and 40.98% (25 of 61) of pancreatic and colorectal cancer tissues. SMO overexpression was significantly associated with smoking, late-stage disease and lymph node metastasis in patients with Colorectal cancer. Our results showed that SMO is dysregulated in pancreatic and colorectal cancers and may be considered as a target in cancer therapeutics.
      (© 2019 Wiley Periodicals, Inc.)
    • Grant Information:
      No. SIMS/Acad/20 of 2015 Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir 190011 India
    • Contributed Indexing:
      Keywords: colorectal cancer; hedgehog; immunohistochemistry; pancreatic cancer; smoothened
    • Publication Date:
      Date Created: 20190221 Latest Revision: 20191120
    • Publication Date:
      20200527
    • Accession Number:
      10.1002/jcb.28477
    • Accession Number:
      30784110
  • Citations
    • ABNT:
      NIYAZ, M. et al. Nuclear localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers. Journal of cellular biochemistry, [s. l.], 2019. DOI 10.1002/jcb.28477. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mnh&AN=30784110. Acesso em: 4 ago. 2020.
    • AMA:
      Niyaz M, Khan MS, Wani RA, Shah OJ, Besina S, Mudassar S. Nuclear localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers. Journal of cellular biochemistry. February 2019. doi:10.1002/jcb.28477.
    • AMA11:
      Niyaz M, Khan MS, Wani RA, Shah OJ, Besina S, Mudassar S. Nuclear localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers. Journal of cellular biochemistry. February 2019. doi:10.1002/jcb.28477
    • APA:
      Niyaz, M., Khan, M. S., Wani, R. A., Shah, O. J., Besina, S., & Mudassar, S. (2019). Nuclear localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers. Journal of Cellular Biochemistry. https://doi.org/10.1002/jcb.28477
    • Chicago/Turabian: Author-Date:
      Niyaz, Madiha, Mosin S Khan, Rauf A Wani, Omar J Shah, Syed Besina, and Syed Mudassar. 2019. “Nuclear Localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers.” Journal of Cellular Biochemistry, February. doi:10.1002/jcb.28477.
    • Harvard:
      Niyaz, M. et al. (2019) ‘Nuclear localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers’, Journal of cellular biochemistry. doi: 10.1002/jcb.28477.
    • Harvard: Australian:
      Niyaz, M, Khan, MS, Wani, RA, Shah, OJ, Besina, S & Mudassar, S 2019, ‘Nuclear localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers’, Journal of cellular biochemistry, viewed 4 August 2020, .
    • MLA:
      Niyaz, Madiha, et al. “Nuclear Localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers.” Journal of Cellular Biochemistry, Feb. 2019. EBSCOhost, doi:10.1002/jcb.28477.
    • Chicago/Turabian: Humanities:
      Niyaz, Madiha, Mosin S Khan, Rauf A Wani, Omar J Shah, Syed Besina, and Syed Mudassar. “Nuclear Localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers.” Journal of Cellular Biochemistry, February 19, 2019. doi:10.1002/jcb.28477.
    • Vancouver/ICMJE:
      Niyaz M, Khan MS, Wani RA, Shah OJ, Besina S, Mudassar S. Nuclear localization and Overexpression of Smoothened in Pancreatic and Colorectal Cancers. Journal of cellular biochemistry [Internet]. 2019 Feb 19 [cited 2020 Aug 4]; Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mnh&AN=30784110