Analysing double-strand breaks in cultured cells for drug screening applications by causal inference

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  • Additional Information
    • Publication Information:
      IEEE
    • Publication Date:
      2018
    • Abstract:
      Double strand breaks (DSB) are a hallmark of DNA damage and genetic instability, which are important features of cancer cells. In addition, repair of DSBs provide interesting therapeutic targets. Fluorescence microscopy allows us to visualise DSBs in cells using a dedicated fluorescent marker, which is therefore an informative readout for drug screening applications. We therefore need robust methods in image analysis and statistical analysis to quantify DSBs in single cells and thereby to assess the drug effect with respect to the related pathways. The contribution of this paper is two-fold: first, we compare different DSB quantification schemes; and second we provide a sound statistical framework based on causal inference in order to detect drugs acting directly on DSBs. In particular this second aspect is so far notoriously neglected in the field, even though it is essential for the specific assignment of the drug effect.
    • Contents Note:
      Conference Acronym: ISBI
    • Author Affiliations:
      MINES Paristech, PSL-Research University, Centre for Computational Biology
      Institut Curie
    • ISBN:
      978-1-5386-3636-7
      978-1-5386-3635-0
    • ISSN:
      1945-8452
    • Relation:
      2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018)
    • Accession Number:
      10.1109/ISBI.2018.8363612
    • Rights:
      Copyright 2018, IEEE
    • AMSID:
      8363612
    • Conference Acronym:
      ISBI
    • Date of Current Version:
      2018
    • Document Subtype:
      IEEE Conference
    • Notes:
      Conference Location: Washington, DC, USA, USA

      Conference Start Date: 4 April 2018

      Conference End Date: 7 April 2018
    • Accession Number:
      edseee.8363612
  • Citations
    • ABNT:
      BOYD, J. et al. Analysing double-strand breaks in cultured cells for drug screening applications by causal inference. 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018), Biomedical Imaging (ISBI 2018), 2018 IEEE 15th International Symposium on, [s. l.], p. 445–448, 2018. DOI 10.1109/ISBI.2018.8363612. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edseee&AN=edseee.8363612. Acesso em: 31 out. 2020.
    • AMA:
      Boyd J, Pinhiero A, Nery ED, Reyal F, Walter T. Analysing double-strand breaks in cultured cells for drug screening applications by causal inference. 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018), Biomedical Imaging (ISBI 2018), 2018 IEEE 15th International Symposium on. April 2018:445-448. doi:10.1109/ISBI.2018.8363612
    • APA:
      Boyd, J., Pinhiero, A., Nery, E. D., Reyal, F., & Walter, T. (2018). Analysing double-strand breaks in cultured cells for drug screening applications by causal inference. 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018), Biomedical Imaging (ISBI 2018), 2018 IEEE 15th International Symposium On, 445–448. https://doi.org/10.1109/ISBI.2018.8363612
    • Chicago/Turabian: Author-Date:
      Boyd, Joseph, Alice Pinhiero, Elaine Del Nery, Fabien Reyal, and Thomas Walter. 2018. “Analysing Double-Strand Breaks in Cultured Cells for Drug Screening Applications by Causal Inference.” 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018), Biomedical Imaging (ISBI 2018), 2018 IEEE 15th International Symposium On, April, 445–48. doi:10.1109/ISBI.2018.8363612.
    • Harvard:
      Boyd, J. et al. (2018) ‘Analysing double-strand breaks in cultured cells for drug screening applications by causal inference’, 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018), Biomedical Imaging (ISBI 2018), 2018 IEEE 15th International Symposium on, pp. 445–448. doi: 10.1109/ISBI.2018.8363612.
    • Harvard: Australian:
      Boyd, J, Pinhiero, A, Nery, ED, Reyal, F & Walter, T 2018, ‘Analysing double-strand breaks in cultured cells for drug screening applications by causal inference’, 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018), Biomedical Imaging (ISBI 2018), 2018 IEEE 15th International Symposium on, pp. 445–448, viewed 31 October 2020, .
    • MLA:
      Boyd, Joseph, et al. “Analysing Double-Strand Breaks in Cultured Cells for Drug Screening Applications by Causal Inference.” 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018), Biomedical Imaging (ISBI 2018), 2018 IEEE 15th International Symposium On, Apr. 2018, pp. 445–448. EBSCOhost, doi:10.1109/ISBI.2018.8363612.
    • Chicago/Turabian: Humanities:
      Boyd, Joseph, Alice Pinhiero, Elaine Del Nery, Fabien Reyal, and Thomas Walter. “Analysing Double-Strand Breaks in Cultured Cells for Drug Screening Applications by Causal Inference.” 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018), Biomedical Imaging (ISBI 2018), 2018 IEEE 15th International Symposium On, April 1, 2018, 445–48. doi:10.1109/ISBI.2018.8363612.
    • Vancouver/ICMJE:
      Boyd J, Pinhiero A, Nery ED, Reyal F, Walter T. Analysing double-strand breaks in cultured cells for drug screening applications by causal inference. 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018), Biomedical Imaging (ISBI 2018), 2018 IEEE 15th International Symposium on [Internet]. 2018 Apr 1 [cited 2020 Oct 31];445–8. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edseee&AN=edseee.8363612