Development of a high density 600K SNP genotyping array for chicken.

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  • Additional Information
    • Publication Year:
      2013
    • Author-Supplied Keywords:
      Chicken
      Genotyping array
      SNP
    • NAICS/Industry Codes:
      NAICS/Industry Codes 112310 Chicken Egg Production
      112340 Poultry Hatcheries
      311615 Poultry Processing
    • Abstract:
      Background: High density (HD) SNP genotyping arrays are an important tool for genetic analyses of animals and plants. Although the chicken is one of the most important farm animals, no HD array is yet available for high resolution genetic analysis of this species. Results: We report here the development of a 600 K AffymetrixW AxiomW HD genotyping array designed using SNPs segregating in a wide variety of chicken populations. In order to generate a large catalogue of segregating SNPs, we re-sequenced 243 chickens from 24 chicken lines derived from diverse sources (experimental, commercial broiler and layer lines) by pooling 10-15 samples within each line. About 139 million (M) putative SNPs were detected by mapping sequence reads to the new reference genome (Gallus_gallus_4.0) of which ~78 M appeared to be segregating in different lines. Using criteria such as high SNP-quality score, acceptable design scores predicting high conversion performance in the final array and uniformity of distribution across the genome, we selected ~1.8 M SNPs for validation through genotyping on an independent set of samples (n = 282). About 64% of the SNPs were polymorphic with high call rates (>98%), good cluster separation and stable Mendelian inheritance. Polymorphic SNPs were further analysed for their population characteristics and genomic effects. SNPs with extreme breach of Hardy-Weinberg equilibrium (P < 0.00001) were excluded from the panel. The final array, designed on the basis of these analyses, consists of 580,954 SNPs and includes 21,534 coding variants. SNPs were selected to achieve an essentially uniform distribution based on genetic map distance for both broiler and layer lines. Due to a lower extent of LD in broilers compared to layers, as reported in previous studies, the ratio of broiler and layer SNPs in the array was kept as 3:2. The final panel was shown to genotype a wide range of samples including broilers and layers with over 100 K to 450 K informative SNPs per line. A principal component analysis was used to demonstrate the ability of the array to detect the expected population structure which is an important pre-investigation step for many genome-wide analyses. Conclusions: This AffymetrixW AxiomW array is the first SNP genotyping array for chicken that has been made commercially available to the public as a product. This array is expected to find widespread usage both in research and commercial application such as in genomic selection, genome-wide association studies, selection signature analyses, fine mapping of QTLs and detection of copy number variants. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • ISSN:
      14712164
    • Accession Number:
      86956723
  • Citations
    • ABNT:
      KRANIS, A. et al. Development of a high density 600K SNP genotyping array for chicken. BMC Genomics, [s. l.], v. 14, n. 1, p. 1, 2013. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=86956723. Acesso em: 25 set. 2020.
    • AMA:
      Kranis A, Gheyas AA, Boschiero C, et al. Development of a high density 600K SNP genotyping array for chicken. BMC Genomics. 2013;14(1):1. Accessed September 25, 2020. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=86956723
    • APA:
      Kranis, A., Gheyas, A. A., Boschiero, C., Turner, F., Le Yu, Smith, S., Talbot, R., Pirani, A., Brew, F., Kaiser, P., Hocking, P. M., Fife, M., Salmon, N., Fulton, J., Strom, T. M., Haberer, G., Weigend, S., Preisinger, R., Gholami, M., & Qanbari, S. (2013). Development of a high density 600K SNP genotyping array for chicken. BMC Genomics, 14(1), 1.
    • Chicago/Turabian: Author-Date:
      Kranis, Andreas, Almas A. Gheyas, Clarissa Boschiero, Frances Turner, Le Yu, Sarah Smith, Richard Talbot, et al. 2013. “Development of a High Density 600K SNP Genotyping Array for Chicken.” BMC Genomics 14 (1): 1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=86956723.
    • Harvard:
      Kranis, A. et al. (2013) ‘Development of a high density 600K SNP genotyping array for chicken’, BMC Genomics, 14(1), p. 1. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=86956723 (Accessed: 25 September 2020).
    • Harvard: Australian:
      Kranis, A, Gheyas, AA, Boschiero, C, Turner, F, Le Yu, Smith, S, Talbot, R, Pirani, A, Brew, F, Kaiser, P, Hocking, PM, Fife, M, Salmon, N, Fulton, J, Strom, TM, Haberer, G, Weigend, S, Preisinger, R, Gholami, M & Qanbari, S 2013, ‘Development of a high density 600K SNP genotyping array for chicken’, BMC Genomics, vol. 14, no. 1, p. 1, viewed 25 September 2020, .
    • MLA:
      Kranis, Andreas, et al. “Development of a High Density 600K SNP Genotyping Array for Chicken.” BMC Genomics, vol. 14, no. 1, Apr. 2013, p. 1. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=86956723.
    • Chicago/Turabian: Humanities:
      Kranis, Andreas, Almas A. Gheyas, Clarissa Boschiero, Frances Turner, Le Yu, Sarah Smith, Richard Talbot, et al. “Development of a High Density 600K SNP Genotyping Array for Chicken.” BMC Genomics 14, no. 1 (April 2013): 1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=86956723.
    • Vancouver/ICMJE:
      Kranis A, Gheyas AA, Boschiero C, Turner F, Le Yu, Smith S, et al. Development of a high density 600K SNP genotyping array for chicken. BMC Genomics [Internet]. 2013 Apr [cited 2020 Sep 25];14(1):1. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=86956723