Drosophila Eggshell Production: Identification of New Genes and Coordination by Pxt.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Subject Terms:
    • Abstract:
      Drosophila ovarian follicles complete development using a spatially and temporally controlled maturation process in which they resume meiosis and secrete a multi-layered, protective eggshell before undergoing arrest and/or ovulation. Microarray analysis revealed more than 150 genes that are expressed in a stage-specific manner during the last 24 hours of follicle development. These include all 30 previously known eggshell genes, as well as 19 new candidate chorion genes and 100 other genes likely to participate in maturation. Mutations in pxt, encoding a putative Drosophila cyclooxygenase, cause many transcripts to begin expression prematurely, and are associated with eggshell defects. Somatic activity of Pxt is required, as RNAi knockdown of pxt in the follicle cells recapitulates both the temporal expression and eggshell defects. One of the temporally regulated genes, cyp18a1, which encodes a cytochromome P450 protein mediating ecdysone turnover, is downregulated in pxt mutant follicles, and cyp18a1 mutation itself alters eggshell gene expression. These studies further define the molecular program of Drosophila follicle maturation and support the idea that it is coordinated by lipid and steroid hormonal signals. [ABSTRACT FROM AUTHOR]
    • :
      Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Department of Anatomy and Cell Biology, Roy J. and Lucille Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America
      2Department of Embryology, Howard Hughes Medical Institute/Carnegie Institution, Baltimore, Maryland, United States of America
    • ISSN:
      1932-6203
    • Accession Number:
      10.1371/journal.pone.0019943
    • Accession Number:
      73816803
  • Citations
    • ABNT:
      TOOTLE, T. L. et al. Drosophila Eggshell Production: Identification of New Genes and Coordination by Pxt. PLoS ONE, [s. l.], v. 6, n. 5, p. 1–12, 2011. DOI 10.1371/journal.pone.0019943. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cxh&AN=73816803. Acesso em: 22 out. 2020.
    • AMA:
      Tootle TL, Williams D, Hubb A, Frederick R, Spradling A. Drosophila Eggshell Production: Identification of New Genes and Coordination by Pxt. PLoS ONE. 2011;6(5):1-12. doi:10.1371/journal.pone.0019943
    • APA:
      Tootle, T. L., Williams, D., Hubb, A., Frederick, R., & Spradling, A. (2011). Drosophila Eggshell Production: Identification of New Genes and Coordination by Pxt. PLoS ONE, 6(5), 1–12. https://doi.org/10.1371/journal.pone.0019943
    • Chicago/Turabian: Author-Date:
      Tootle, Tina L., Dianne Williams, Alexander Hubb, Rebecca Frederick, and Allan Spradling. 2011. “Drosophila Eggshell Production: Identification of New Genes and Coordination by Pxt.” PLoS ONE 6 (5): 1–12. doi:10.1371/journal.pone.0019943.
    • Harvard:
      Tootle, T. L. et al. (2011) ‘Drosophila Eggshell Production: Identification of New Genes and Coordination by Pxt’, PLoS ONE, 6(5), pp. 1–12. doi: 10.1371/journal.pone.0019943.
    • Harvard: Australian:
      Tootle, TL, Williams, D, Hubb, A, Frederick, R & Spradling, A 2011, ‘Drosophila Eggshell Production: Identification of New Genes and Coordination by Pxt’, PLoS ONE, vol. 6, no. 5, pp. 1–12, viewed 22 October 2020, .
    • MLA:
      Tootle, Tina L., et al. “Drosophila Eggshell Production: Identification of New Genes and Coordination by Pxt.” PLoS ONE, vol. 6, no. 5, May 2011, pp. 1–12. EBSCOhost, doi:10.1371/journal.pone.0019943.
    • Chicago/Turabian: Humanities:
      Tootle, Tina L., Dianne Williams, Alexander Hubb, Rebecca Frederick, and Allan Spradling. “Drosophila Eggshell Production: Identification of New Genes and Coordination by Pxt.” PLoS ONE 6, no. 5 (May 2011): 1–12. doi:10.1371/journal.pone.0019943.
    • Vancouver/ICMJE:
      Tootle TL, Williams D, Hubb A, Frederick R, Spradling A. Drosophila Eggshell Production: Identification of New Genes and Coordination by Pxt. PLoS ONE [Internet]. 2011 May [cited 2020 Oct 22];6(5):1–12. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cxh&AN=73816803