The expression profile of purified Drosophila germline stem cells

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  • Additional Information
    • Author-Supplied Keywords:
      bone morphogenetic protein ( BMP )
      Drosophila
      Expression profile
      fluorescence-activated cell sorting ( FACS )
      Germ cell
      germline stem cell ( GSC )
      human telomerase RNA ( hTR )
      Polycomb repressive complex 1 ( Prc1 )
      primordial germ cell ( PGC )
      propidium diiodide ( PI )
      Psc
      RpL22
      Stem cell
      TATA-associated factors ( TAFs )
      TATA-binding proteins ( Tbps )
    • Abstract:
      Abstract: We developed a method to highly purify germline stem cells (GSCs) from the Drosophila ovary, one of the best understood types of adult stem cell. GSCs express variant isoforms of general transcriptional components, translation initiation factors, and several variant ribosomal proteins, including RpL22, a protein enriched in several mammalian stem cells. These novel isoforms may help regulate stem cell gene expression because a reversion assay indicated that at least four were specific for GSCs. By comparative analysis, we identify additional genes enriched in GSCs, including Psc, the Drosophila homolog of the Bmi-1 Polycomb group gene, as well as genes that may delay cytokinesis in pre-meiotic germ cells. By comparing GSCs arrested by BMP over-expression and bam mutation, we hypothesize that mRNA utilization is modulated in differentiating GSC daughters. Our findings suggest that Drosophila and mammalian stem cells utilize at least two regulatory mechanisms in common. [Copyright &y& Elsevier]
    • :
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    • Author Affiliations:
      1Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution, 115 West University Parkway, Baltimore, MD 21210, USA
    • ISSN:
      0012-1606
    • Accession Number:
      10.1016/j.ydbio.2005.04.018
    • Accession Number:
      18252011
  • Citations
    • ABNT:
      KAI, T.; WILLIAMS, D.; SPRADLING, A. C. The expression profile of purified Drosophila germline stem cells. Developmental Biology, [s. l.], v. 283, n. 2, p. 486–502, 2005. DOI 10.1016/j.ydbio.2005.04.018. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asr&AN=18252011. Acesso em: 20 out. 2020.
    • AMA:
      Kai T, Williams D, Spradling AC. The expression profile of purified Drosophila germline stem cells. Developmental Biology. 2005;283(2):486-502. doi:10.1016/j.ydbio.2005.04.018
    • APA:
      Kai, T., Williams, D., & Spradling, A. C. (2005). The expression profile of purified Drosophila germline stem cells. Developmental Biology, 283(2), 486–502. https://doi.org/10.1016/j.ydbio.2005.04.018
    • Chicago/Turabian: Author-Date:
      Kai, Toshie, Dianne Williams, and Allan C. Spradling. 2005. “The Expression Profile of Purified Drosophila Germline Stem Cells.” Developmental Biology 283 (2): 486–502. doi:10.1016/j.ydbio.2005.04.018.
    • Harvard:
      Kai, T., Williams, D. and Spradling, A. C. (2005) ‘The expression profile of purified Drosophila germline stem cells’, Developmental Biology, 283(2), pp. 486–502. doi: 10.1016/j.ydbio.2005.04.018.
    • Harvard: Australian:
      Kai, T, Williams, D & Spradling, AC 2005, ‘The expression profile of purified Drosophila germline stem cells’, Developmental Biology, vol. 283, no. 2, pp. 486–502, viewed 20 October 2020, .
    • MLA:
      Kai, Toshie, et al. “The Expression Profile of Purified Drosophila Germline Stem Cells.” Developmental Biology, vol. 283, no. 2, July 2005, pp. 486–502. EBSCOhost, doi:10.1016/j.ydbio.2005.04.018.
    • Chicago/Turabian: Humanities:
      Kai, Toshie, Dianne Williams, and Allan C. Spradling. “The Expression Profile of Purified Drosophila Germline Stem Cells.” Developmental Biology 283, no. 2 (July 15, 2005): 486–502. doi:10.1016/j.ydbio.2005.04.018.
    • Vancouver/ICMJE:
      Kai T, Williams D, Spradling AC. The expression profile of purified Drosophila germline stem cells. Developmental Biology [Internet]. 2005 Jul 15 [cited 2020 Oct 20];283(2):486–502. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asr&AN=18252011